Kinetics of the reaction between trypsin and the pancreatic trypsin inhibitor
نویسندگان
چکیده
منابع مشابه
Pancreatic Trypsin Inhibitor 2. REACTION WITH TRYPSIN BY N. M. GREEN* AND ELIZABETH WORK
The reactions of trypsin with its inhibitors are among the few known cases of specific interaction between two purified proteins (Kunitz & Northrop, 1936; Kunitz, 1947; Fraenkel-Conrat, Bean & Lineweaver, 1949; Fraenkel-Conrat, Bean, Ducay & Olcott, 1952; Schmitz, 1939; Laskowski & Laskowski, 1951). Under favourable conditions, the inhibitor-trypsin compound is formed extremely easily; thus, we...
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1. The rate of hydrolysis of a casein solution by trypsin is not affected by the addition of gelatin. The trypsin, therefore, is not combined with the gelatin unless there is a separate enzyme for casein and for gelatin. 2. The presence of casein protects the gelatin-splitting power of trypsin from heat inactivation, and the presence of gelatin protects the casein-splitting power from heat inac...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1957
ISSN: 0306-3283
DOI: 10.1042/bj0660407